Cytogenetcs
Irradiation and radiosensitivity
Peripheral blood lymphocytes are ideal cells for the analysis of chromosomal aberrations in conditions of suspected overexposure to ionizing radiation.
The absorbed dose of ionizing radiation can be estimated within 2-3 days.
Using blood sample it is also possible to estimate intrinsic (genomic) radiosensitivity, which is used to diagnose chromosomal instability disorders or as a predictive test in radiotherapy.
Irradiation
BIOMARKERs of DNA double-chain breaks |
Damages |
consequences |
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Numerous enzymes (more than 80) are involved in DNA damage recovery – the so-called "repair enzymes" that clean up damage to the genome, to reduce potential problems. If the genome is heavily damaged, further damages may arise during repair, resulting in rearrangements and mutations. |
These rearrangements do not have negative effect on the phenotype, but do affect and have negative consequences for the offsprings.
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Chromosome aberrations |
Micronuclei |
